Development of a novel method to detect infectious viruses from reclaimed wastewater based on apoptosis of host cultivated cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K12635
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental risk control and evaluation
• Research Institution: Tohoku University (2017); Hokkaido University (2016)
• Principal Investigator: Sano Daisuke 東北大学, 工学研究科, 准教授 (80550368)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 胃腸炎ウイルス / 感染性 / 細胞応答 / アポトーシス / 感染リスク

Outline of Final Research Achievements

This study aimed to develop a novel method to detect infectious enteric viruses in water samples. In the first year, cellular responses, including apoptosis, in the early stage of enteric virus infection with human cultivated cells were investigated as possible indicator for the virus detection. As a result, it was shown that infectious enterovirus 71 (EV71) was possible to detect within 12 hours when the concentration in a inoculated sample was high enough. In the second year, the effect of multiple virus infection with host cells on the early detection of infectious viruses was investigated. As a result, the detection of adenovirus 40 (AdV40) was significantly inhibited by the co-infection of EV71. This result implies that the inhibition of EV71 growth is necessary when AdV40 is a target virus in water samples.

Free Research Field

環境水質工学