2017 Fiscal Year Final Research Report
Study for food factors inducing immunostimulating activity
| Project/Area Number |
16K12726
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits
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| Research Institution | University of Shizuoka |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 機能性食品 |
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| Outline of Final Research Achievements |
Although it has been recently reported that Arabidopsis thaliana contains nuclear genes encoding orthologs of key enzymes of bacterial lipid A biosynthesis, the end product of lipid A biosynthesis pathway in plant is not identified. In this study, we performed experiments to identify novel lipid A-like molecules in Arabidopsis thaliana and evaluate the imunostimulating activities. The seedings of Arabidopsis were treated to extract the fraction including lipid A, which was then applied to thin layer chromatography (TLC) and mass spectrometric (MS) analysis. Some unidentified molecules were detected in TLC and MS analysis. Also, mRNA expression of cytokines in human monocyte THP-1 cell after the treatment with extracted lipid A fraction were evaluated.
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| Free Research Field |
食品機能学
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