Effects of Atmospheric-pressure plasma irradiation on insulin-target cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K12863
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tohoku University
• Principal Investigator: Kanzaki Makoto 東北大学, 医工学研究科, 准教授 (10272262)
• Co-Investigator(Renkei-kenkyūsha): Kaneko Toshiro 東北大学, 大学院工学研究科, 教授 (30312599)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 大気圧プラズマ / 細胞

Outline of Final Research Achievements

Recently, non-equilibrium atmospheric pressure plasma (APP) emerged to exert diverse biological responses, though the underlying mechanisms remained to be determined. This study aimed to explore precise mechanisms underlying of plasma-inducible increases in intracellular Ca2+ concentration in insulin-responsive cells, which has been recently discovered by our research group. Our results demonstrated that increases in intracellular Ca2+ are induced by continuous low-dose O2－/ ONOO－ supply, which is mediated through TRP channel activation. Our findings indicate the potential of APP for regulating insulin-responsive cell functions. Moreover, APP showed inhibitory actions on adipogenic differentiation of 3T3L1 cells. Together, these results demonstrate that APP-induced TRP channel activation is intimately involved in various cellular responses triggered by APP exposure.

Free Research Field

分子糖尿病学