Effects of polyunsaturated fatty acids supplemented in medium on primary culture

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K12864
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Yamagata University
• Principal Investigator: NAKAMURA TAKAO 山形大学, 大学院医学系研究科, 教授 (00142654)
• Co-Investigator(Renkei-kenkyūsha): KUSUNOKI Masataka 名古屋大学, 総合保健体育科学センター, 教授 (80214956) ; SATO Daisuke 山形大学, 大学院医学系研究科, 助教 (60536960)
• Research Collaborator: FENG Zhonggang ; TAKAHASHI Kazuaki ; SASAKI Hiroyuki ; KOSAKA Kaoru ; IGUCHI Seiya ; SAITO Jo ; TAKAHASHI Shinya ; OTORI Maki ; YANO Mizuna
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 再生医工学 / 脂質 / 初代培養 / 心筋細胞 / 脂肪細胞

Outline of Final Research Achievements

Primary culture of rat cardiomyocytes was conducted, using medium containing one of 5 fatty acids including docosahexaenoic (DHA) and arachidonic (AA) acids. Maximal contractile performance was obtained in cases of 20μM DHA and 50μA AA, with large difference in gene expression. Although combined supplementation of 10μM DHA and 10-20μM AA showed maximal performance of contractility, its level stayed rather close to that obtained in single DHA supplementation.
Primary culture of adult rat adipocytes was superior to that of juvenile one. However, even in the case of DHA supplementation, retaining of adipocytes remained difficult. These results may show effects of fatty acids are dependent on cell species.

Free Research Field

生体医工学