Quantification of failure conditions of a single red blood cell by non-contact mechanical tests using MEMS and chemiluminescene reactions

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K12867
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Nagoya Institute of Technology (2017); Saitama University (2016)
• Principal Investigator: Nakamura Masanori 名古屋工業大学, 工学(系)研究科(研究院), 教授 (20448046)
• Co-Investigator(Kenkyū-buntansha): 木村 直行 自治医科大学, 医学部, 准教授 (20382898) ; 吉川 洋史 埼玉大学, 理工学研究科, 准教授 (50551173)
• Research Collaborator: YAGI Takanobu 早稲田大学, 理工学術院, 研究院准教授 (00468852)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 赤血球 / 溶血 / 破壊条件

Outline of Final Research Achievements

The present study attempted to determine the fracture strain of a single red blood cell (RBC) using non-contact mechanical tests. A crossed microchannel with a width of 50 um at the junction was fabricated. RBCs were introduced to the microchannel with expectations that RBCs are instantaneously stretched at the junction by colliding flows. The deformation behavior was observed with a microscope and recorded with a high speed camera. The amount of residual hemoglobin was estimated from the collected red blood cells based on the Lambert- Beer's law. Correlation between the maximum amount of deformation of RBCs during flow and the amount of residual hemoglobin was examined, and no correlation was found. On the other hand, a negative correlation was found between the compressive deformation rate and the residual amount of hemoglobin. These results suggested that RBSs are damaged more by compression than stretch.

Free Research Field

生体医工学