2017 Fiscal Year Final Research Report
Development of a microfluidic device to mimic the function of the thymus for immunotherapy
| Project/Area Number |
16K12874
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
金子 新 京都大学, iPS細胞研究所, 准教授 (40361331)
三嶋 雄太 京都大学, iPS細胞研究所, 特定研究員 (80770263)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | Organ-on-a-chip / マイクロ流体デバイス / iPS細胞 / 胸腺 / 血管 / T細胞 / 3次元培養 |
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| Outline of Final Research Achievements |
The goal of this study is to develop a culture device to mimic the function of the thymus which is necessary to form functional T cells. Using human induced pluripotent stem cell (hiPSC)-derived endothelial cells, we have successfully engineered a 3D vascular network in a hydrogel embedded within a microfluidic device. The engineered vascular network has open lumina connected to microchannels, which can deliver reagents and cells. Using this technique, we recapitulated a thymus-like microenvironment by culturing 3D cellular constructs containing thymus-like stromal cells with hiPSC-derived endothelial cells. This system enabled to perfuse blood cells through blood vessels connected to the tissue-like 3D cell constructs and to maintain blood cells inside the device. Thus, we have successful developed a microfluidic device to mimic the structure and microenvironment of the thymus.
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| Free Research Field |
生体医工学
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