Development of fluorescence lifetime measurement for glycation screening for diabetes in vivo

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K12877
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Osaka University
• Principal Investigator: Miura Jiro 大阪大学, 歯学部附属病院, 助教 (70437383)
• Co-Investigator(Kenkyū-buntansha): 橋本 守 北海道大学, 情報科学研究科, 教授 (70237949)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 糖化 / メイラード反応 / 蛍光寿命

Outline of Final Research Achievements

Glycation, namely the Maillard reaction, is a non-enzymatic reaction between protein or lipid and reducing sugar, resulting in formation of advanced glycation end-products (AGEs). Because glycation of collagen progresses under physiological conditions in living organisms, glycation is one of the most important processes in human aging. Several AGEs bind collagen and act as cross-links between collagen fibrils, thereby changing the fluorescent lifetime of collagen-rich tissues. The purpose of this study is to identify AGEs in human dentin using electron microscopy, time-resolved fluorescence microscopy, mechanical testing and chemical analyses. Our findings suggest the potential influence of cross-linking on diabetic and aging tissue.

Free Research Field

生体工学