2017 Fiscal Year Final Research Report
Controlling cell fates of iPS cells on growth factor-immobilized hydrogel by mechanical strain
| Project/Area Number |
16K12879
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
水本 博 九州大学, 工学研究院, 准教授 (90346817)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 幹細胞 / 分化 / ハイドロゲル / 増殖因子 / メカノトランスダクション |
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| Outline of Final Research Achievements |
This research attempted to combine the advantages of the control of growth factors and the adjustable mechanical properties of the scaffold, to provide variety choice for scaffold design. We focus on the heparin as a growth factor-immobilization agent, and we prepared heparin-conjugated collagen gel. We also built crosslink between the collagen fibers to change the mechanical properties. By using heparin-conjugated collagen gel and immobilizing some growth factors, the survival and functions of cultured cells were up-regulated. On the other hand, the research on the change of the mechanical properties, which could cause by the cross-linking level of the substrate itself, showed the influence on the state of the cells. In the future, this study would contribute to the investigation of the mechanisms underlying cell-fate decisions.
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| Free Research Field |
化学工学・高分子化学
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