Chemical biology of multifunctional transporter GTR1

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K13083
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Tohoku University
• Principal Investigator: Ueda Minoru 東北大学, 理学研究科, 教授 (60265931)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 植物ホルモン輸送体 / GTR1 / NPR family

Outline of Final Research Achievements

Spatiotemporal distributions of plant hormones are regulated strictly through corresponding transporter proteins. GTR1 was found first found as a transporter of a jasmonoyl isoleucine (JA-Ile) and belongs to a NPF family to which 53 member proteins belong. Surprisingly, further studies strongly suggested that GTR1 is a unique `multi-functional` transporter which can transport three different kinds of plant metabolites, JA-Ile, gibberelin, and glucosinolate, across the membrane with structure recognition. We also found that the homo-dimerization of GTR1 is responsible for glucosinolate transport and heterodimerization with other NPF family protein is responsible for the transport of JA-Ile and GA. This is the first example of dimerization-mediated switching of substrate for transporter.

Free Research Field

ケミカルバイオロジー、天然物化学