Development of protein-labeling probes with OFF-ON-OFF fluorescence switches

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K13088
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Osaka University
• Principal Investigator: Hori Yuichiro 大阪大学, 工学研究科, 准教授 (00444563)
• Research Collaborator: NISHIURA Miyako ; HIRAYAMA Shinya ; OTOMURA Norimichi ; NISHIDA Ayuko ; MORI Kazuma ; YAMASAKI Kohei ; UMENO Maho
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: OFF-ON-OFFプローブ / PYPタグ / 蛋白質分解

Outline of Final Research Achievements

In this research, we developed protein labeling probes for imaging protein lifetime using our original PYP-tag labeling method and a new chemical principle based on OFF-ON-OFF fluorescence switching. The regulation of intramolecular association made the probes nonfluorescent in a free state, fluorescent in a PYP-tag-bound form and nonfluorescent after protein degradation. By using one of the probes, we imaged protein expression and degradation in living cells.

Free Research Field

ケミカルバイオロジー、蛍光イメージング