Reprogramming of root development by diffusible signals produced during symbiotic interaction of Arabidopsis and a root endophytic fungus Serendipita indica.

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K13090
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Osaka Prefecture University
• Principal Investigator: Ohta Daisaku 大阪府立大学, 生命環境科学研究科, 教授 (10305659)
• Co-Investigator(Renkei-kenkyūsha): Akiyama Kohki 大阪府立大学, 生命環境科学研究科, 教授 (20285307)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 植物共生菌 / 生育促進 / 側根原基誘導 / オーキシン生合成

Outline of Final Research Achievements

Upon colonization by Serendipita indica, an endophytic fungus, root development of the host plant Arabidopsis is greatly affected: primary root growth is strongly inhibited, while lateral root (LR) initiation is remarkably stimulated. We found that unidentified diffusible substance(s) produced during the endophytic interaction elevated local of auxin levels at specific sites of the primary root followed by the increased frequency of LR initiation. Reverse genetics and chemical biology studies have demonstrated that the LR development and the local auxin production were independent of the known auxin biosynthetic pathways in Arabidopsis. Toward understanding the molecular mechanisms underlying the growth promotion during the endophytic interaction, identification and characterization of the diffusible substances and the unclarified auxin biosynthetic pathway are now underway.

Free Research Field

応用分子生物学