Cultivation of Useful Marine Cyanobacteria and Analysis of Biosynthetic genes

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K13091
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Keio University
• Principal Investigator: Suenaga Kiyotake 慶應義塾大学, 理工学部(矢上), 教授 (60273215)
• Co-Investigator(Renkei-kenkyūsha): Iwasaki Arihiro 慶應義塾大学, 理工学部, 助教 (00754897) ; Kudo Fumitaka 東京工業大学, 理工学部研究科, 准教授 (00361783)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 海洋シアノバクテリア / カナミエナミド / エノールエーテル / リングビアペプチンB / 全ゲノム増幅 / 全合成 / 生物活性

Outline of Final Research Achievements

Whole genome amplification of marine cyanobacteria Moorea bouillonii which produces kanamienamide were conducted. Amplified genome DNA had good quality and purity, its sequence analysis was performed and biosynthetic gene clusters of lyngbyapeptin B with enol ether moiety were found. Enzymatic reaction with model substrates have been investigated. In addition, total synthesis of kanamienamide was achieved. The several assays of synthetic kanamienamide revealed its real biological activity

Free Research Field

天然物化学