2016 Fiscal Year Final Research Report
Fluorescence screening and imaging by chemical probes for detecting histone deacetylases/demethylases activity
Project/Area Number |
16K13099
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Chemical biology
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Research Institution | Osaka University |
Principal Investigator |
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Project Period (FY) |
2016-04-01 – 2017-03-31
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Keywords | 蛍光プローブ / ヒストン脱メチル化酵素 / エピジェネティクス |
Outline of Final Research Achievements |
Fluorescent probes were developed for detection of lysine demethylase (KDM) activity. This fluorescent probe produced a fluorescent coumarin upon lysine demethylation by utilizing the intramolecular transesterification mechanism. As results, KDM activity was successfully detected with a single-step manipulation for the first time. The probe will be useful for understanding the role of KDM and contribute to development of drugs targeting KDM activity.
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Free Research Field |
ケミカルバイオロジー
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