Extraction of neurite signals from neuropil calcium signaling

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K13116
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Brain biometrics
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Hirsae Hajime 国立研究開発法人理化学研究所, 脳科学総合研究センター, チームリーダー (90392084)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: ニューロピル / GCaMP / イメージング

Outline of Final Research Achievements

Neuropil Ca2+ dynamics has been imaged using in in vivo two-photon microscopy from the visual cortex of the G7NG817 transgenic mouse, in which astrocytes and a subpopulation of neurons express the Ca2+ sensor G-CAMP7. G7NG817 mice has been crossed with an Alzheimer’s disease model mouse line, from which similar neuropil imaging has been performed. The data were analyzed with a non-negative matrix factorization-assisted algorithm that automatically extracted individual Ca2+ active regions. Post-hoc examination of these regions showed that some e regions contained fast transients and some other regions exhibited slow transients. These are postulated to correspond to synaptopil and gliopil, respectively.

Free Research Field

神経科学