2017 Fiscal Year Final Research Report
Extraction of neurite signals from neuropil calcium signaling
Project/Area Number |
16K13116
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Brain biometrics
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Hirsae Hajime 国立研究開発法人理化学研究所, 脳科学総合研究センター, チームリーダー (90392084)
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Project Period (FY) |
2016-04-01 – 2018-03-31
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Keywords | ニューロピル / GCaMP / イメージング |
Outline of Final Research Achievements |
Neuropil Ca2+ dynamics has been imaged using in in vivo two-photon microscopy from the visual cortex of the G7NG817 transgenic mouse, in which astrocytes and a subpopulation of neurons express the Ca2+ sensor G-CAMP7. G7NG817 mice has been crossed with an Alzheimer’s disease model mouse line, from which similar neuropil imaging has been performed. The data were analyzed with a non-negative matrix factorization-assisted algorithm that automatically extracted individual Ca2+ active regions. Post-hoc examination of these regions showed that some e regions contained fast transients and some other regions exhibited slow transients. These are postulated to correspond to synaptopil and gliopil, respectively.
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Free Research Field |
神経科学
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