Elucidation of antimicrobial mechanism by silver nanoparticles

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K13628
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nanomaterials chemistry
• Research Institution: Osaka University
• Principal Investigator: Seino Satoshi 大阪大学, 工学研究科, 准教授 (90432517)
• Research Collaborator: IMOTO Yasuo ; NISHIDA Tomoki
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 銀ナノ粒子 / 抗微生物

Outline of Final Research Achievements

Antimicrobial mechanism of silver nanoparticles were discussed by using material analysis methods. The XAFS analysis technique was used to investigate the change in the chemical state of silver nanoparticles under antimicrobial test environments. Sulfur-containing substances such as cysteine, methionine and glutathione was used as model compounds. It was revealed that chemical state of the silver nanoparticles changed from the metal to form sulfur bonded state under high concentration of sulfur-containing substances. It was strongly suggested that this change in the chemical state is a factor to inactivate antimicrobial properties.
SEM observation of antibacterial treated bacteria was carried out using E-coli as the model bacterial species. It was revealed that the action mechanism of silver nanoparticles was not membrane destruction.

Free Research Field

ナノ粒子工学