Creation of non-spherical liposomes using a microdevice

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K13656
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nano/Microsystems
• Research Institution: Kanagawa Institute of Industrial Sclence and Technology (2017); Kanagawa Academy of Science and Technology (2016)
• Principal Investigator: Kamiya Koki 地方独立行政法人神奈川県立産業技術総合研究所, 人工細胞膜システムグループ, 研究員(任期有) (70612315)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: リポソーム / 細胞模倣膜 / 人工細胞モデル / マイクロデバイス / 非球体リポソーム

Outline of Final Research Achievements

I developed a cell-sized non-spherical liposome formation using three-dimensional micro structures to emulate a surface structure of cultured cells. I fabricated a micro-cubic frame based on a stackable photoresist processed using two photon laser writing. Next, I formed lipid membranes on the faces of the micro-cubic frame structures into microfluidic devices. Moreover, to confirm the formation of the lipid bilayer membranes on the faces of the micro-cubic frame structures, nanopore-forming membrane proteins were reconstituted into the non-spherical liposomes. I observed the diffusion of fluorescent molecules through the nanopore-forming membrane proteins.

Free Research Field

生体関連化学、生物物理学