Development of super-photostable fluorescent dye library

2016 Fiscal Year Final Research Report

• Project/Area Number: 16K13949
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Organic chemistry
• Research Institution: Nagoya University
• Principal Investigator: Yamaguchi Shigehiro 名古屋大学, 物質科学国際研究センター(WPI), 教授 (60260618)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Keywords: π共役化合物 / 蛍光イメージング / 耐光性 / リン / 有機蛍光色素

Outline of Final Research Achievements

In the current fluorescence imaging in biology, one of the key issues from the materials side is to improve photostability of organic fluorescent dyes. We have achieved the synthesis of highly photostable fluorescent molecules with ladder-type phosphorus-containing skeletons and elucidated the impact of the bridging moiety that makes the pi-skeleton rigid. With the photostable dye in hand, we have achieved the repeated observation of subcellular structures by super-resolution stimulated emission depletion (STED) microscopy.

Free Research Field

合成化学