Development of Anti-Freeze Glycoprotein

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K13992
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Synthetic chemistry
• Research Institution: Nagoya Institute of Technology
• Principal Investigator: Shibata Norio 名古屋工業大学, 工学(系)研究科(研究院), 教授 (40293302)
• Research Collaborator: TOKUNAGA Etsuko 名古屋工業大学, 大学院工学研究科, 特任研究員 (70569889) ; SUMII Yuji 名古屋工業大学, 大学院工学研究科, 助教 (10612848) ; SAIDALIMU YIWUREYIMUJIANG 名古屋工業大学, 大学院工学研究科, 外国人特別研究員
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: フッ素 / ペプチド / プロリン / 糖 / 不凍活性

Outline of Final Research Achievements

Antifreeze (glyco)proteins are very large molecules found in the blood serum of Antarctic notothenioid fish and Arctic cod. While the utility of antifreeze (glyco)proteins has been expected in a variety of area in life sciences, the availability of antifreeze (glyco)proteins is strictly limited to natural resources. We herein report the design and synthesis of antifreeze (glyco)proteins with small molecular weights. Based on our novel hypothesis, "a marbled beef hypothesis", we designed a (glyco)protein containing fluorinated amino acids and sugars, i.e., galactose nona-fluorinated proline (Gal-F-Pro9) as a material which demonstrates the hypothesis. The synthesis of Gal-F-Pro9 was achieved by the Click reaction of nona-fluorinated proline and propargyl-galactose. As our expectation, Gal-F-Pro9 showed an inhibition of ice recrystallization.

Free Research Field

フッ素化学