development of multi-wavelength periodic illumination technique for super-resolution microscopy

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14020
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: Osaka University
• Principal Investigator: Yoshiki Nakata 大阪大学, レーザーエネルギー学研究センター, 准教授 (70291523)
• Research Collaborator: SAITO Tetsuya 大阪大学, 大学院工学研究科
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 超解像顕微鏡 / STED顕微鏡 / 構造化照明顕微鏡 / 干渉 / 位相 / 励起 / 脱励起 / レーザー

Outline of Final Research Achievements

In this experiment, the base technologies to combine STED super-resolution microscope and SIM microscope were developed. First, co-axial laser (λ=405nm & 488nm) was composed, then interference patterns at these wavelength with the same period Λ=126.0 micron was formed by a diffractive optical element and a Fourier optics. Second, the relative position of these interference patterns were controlled by means of wavelength dispersion of a glass plane. Third, double stage Fourier transfer systems was composed to avoid stray light. Then, interference patterns and images of fluorescent particles were imaged by the microscope with structured illumination.

Free Research Field

レーザー工学