PCR based on Surface Plasmon Enhanced Site-selective Heating

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14022
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: Kyoto Prefectural University
• Principal Investigator: Ishida Akito 京都府立大学, 生命環境科学研究科, 教授 (20184525)
• Research Collaborator: Kawahara Yuki 京都府立大学, 大学院生命科学研究科・応用生命科学専攻, 大学院生
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 表面プラズモン共鳴 / プラズモン増強加熱 / 光PCR / 等温連続DNA増幅反応 / １分子加熱 / 蛍光免疫分析 / 病原体計数 / 新興感染症対策

Outline of Final Research Achievements

In this study, site-selective enzymatic DNA elongation from gold surface at 25°C was induced by the damping heat of propagating surface plasmon (SP), using an 808 nm laser. A multispot immunoassay, based on this concept, has been demonstrated. The primer-immobilized analyte-bound ligand spot was selectively heated by the SP field, and effective DNA elongation on the surface using rolling circle amplification produced an intense fluorescence spot on the surface after staining. The key feature of this method is the temperature contrast on the surface. We have estimated the surface temperature by comparison of the yield and chain length of the amplicons from SP heating to those from controlled thermal reactions at a series of temperatures.

Free Research Field

蛍光分光化学