Development of optogenetics tools regulated by light with longer wavelength

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14030
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Osaka University (2017); University of Fukui (2016)
• Principal Investigator: Hattori Mitsuru 大阪大学, 産業科学研究所, 特任助教(常勤) (20589858)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 光遺伝学 / 蛍光観察 / 蛍光タンパク質 / 光応答タンパク質

Outline of Final Research Achievements

We tried to establish a new optogenetics tool to regulate a protein in living cells by light with longer wavelength. For the purpose, near-field light from fluorescent molecules or photo-reactive proteins regulated by longer wavelength light were used.
In the method with photo-reactive proteins, we considered with a regulation of protein function by light. Ion-pump protein on cell membrane was selected, which was regulated by 580 nm light. The relationship between photo-reaction and variation of cell function were investigated with chemiluminescent protein sensor. In the result, cell phenomenon reacted with photo-irradiation were confirmed. Finally, we identified the possibility of photo-reactive proteins with the optical function with longer wavelength light.

Free Research Field

分子生物学