Development of cross-reactive molecule libraries for generating information on antibody misfolding

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14043
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Shunsuke Tomita 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 研究員 (50726817)
• Co-Investigator(Kenkyū-buntansha): 白木 賢太郎 筑波大学, 数理物質系, 教授 (90334797)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 抗体 / バイオセンサー / 酸化グラフェン / DNA / 多変量解析 / 高分子

Outline of Final Research Achievements

In recent years, antibodies have been widely used for medical purposes, but there is a problem that antibodies tend to degrade during purification, transportation and storage processes. Therefore, when designing therapeutic antibodies, it is necessary to strictly determine the methods and conditions of antibody processing and storage in each process based on a combination of separation and spectroscopic analyses. In this project, a novel sensing method was developed to address these issues. By using cross-reactive complexes between fluorescent DNAs and nanosized-graphene oxides (nGOs), the information on antibody misfolding was transduced into response fingerprints, and multivariate analyses of these fingerprints allowed for rapid and facile identification of the degraded states of the antibodies.

Free Research Field

バイオ分析化学