Proposal of cell-mimetic platform for membrane protein function analysis using ISFET

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14107
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Device related chemistry
• Research Institution: NTT Basic Research Laboratories
• Principal Investigator: Kashimura Yoshiaki 日本電信電話株式会社NTT物性科学基礎研究所, 機能物質科学研究部, 主任研究員 (90393751)
• Co-Investigator(Kenkyū-buntansha): 大嶋 梓 日本電信電話株式会社NTT物性科学基礎研究所, 機能物質科学研究部, 研究員 (90751719)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: ナノバイオ / 生体機能利用 / タンパク質 / 人工生体膜 / ISFET

Outline of Final Research Achievements

The aim of this research is to provide a platform for the membrane protein analysis, where the function of membrane proteins is detected not directly by measuring the ion channel current, but by utilizing cell-mimetic microwells equipped with ion sensitive field effect transistors. In this research period, FET was fabricated on silicon substrate, and fabrication of microwell structure consisting of SiN/SiO2, which has the same size as a cell, was made on top of FET. Furthermore, theoretical calculations were performed on the microwell structure sealed with the lipid membrane. We obtained useful knowledge about the optimal combination of the substrate surface and the lipid membrane composition in this device structure.

Free Research Field

ナノバイオテクノロジー