Development of In-Cell Imaging Technique for Studying Molecular Dynamics in Living Cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14132
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Production engineering/Processing studies
• Research Institution: Toyohashi University of Technology
• Principal Investigator: SHIBATA TAKAYUKI 豊橋技術科学大学, 工学(系)研究科(研究院), 教授 (10235575)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 走査型プローブ顕微鏡 / チップ増強ラマン分光法 / 細胞機能解析 / 細胞操作 / バイオMEMS

Outline of Final Research Achievements

With the aim of developing an in-cell imaging technique for studying the dynamic behavior of biological molecules inside cells with high spatial and temporal resolution, we developed a fabrication process for a flat rectangular fluidic microchannel embedded into a Si cantilever beam structure for the geometric optimization of a newly designed AFM probe we call a "bioprobe" that is integrated with a sharp-tipped hollow nanoneedle instead of a conventional solid tip. Moreover, we demonstrated the possibility of utilizing tip-enhanced Raman spectroscopy (TERS) for enabling quantitative study on molecular dynamic processes inside single living cells with the homemade Raman system.

Free Research Field

MEMS，マイクロ・ナノ加工