Measurement of bactericidal effects by reactive species for sterilization using plasma

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14208
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Power engineering/Power conversion/Electric machinery
• Research Institution: The University of Tokyo
• Principal Investigator: Ono Ryo 東京大学, 大学院新領域創成科学研究科, 准教授 (90323443)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: sterilization / discharge plasma / reactive species / vacuum ultraviolet / photolysis / plasma medicine

Outline of Final Research Achievements

In the sterilization using discharge plasma including plasma medicine and water treatment, reactive species such as OH radicals are considered to cause the sterilization. However, the bactericidal effects by those short-lived reactive species including OH radicals have not been directly measured. In this study, we quantitatively measured the bactericidal effects by OH radicals, ozone, and H2O2 on Bacillus subtilis and Escherichia coli using the method we have developed, which selectively produces specific types of reactive species using photolysis by vacuum ultraviolet light. Then, we compared the bactericidal effects by OH, ozone, and H2O2, and the bactericidal effects on the two types of bacteria. In addition, we measured the densities of reactive species produced by this method to verify the reliability of the simulation which calculates the densities of reactive species of this method.

Free Research Field

プラズマ応用工学