Fabrication and development of biosensor to detect aggregation of amyloid-beta oligomer

2016 Fiscal Year Final Research Report

• Project/Area Number: 16K14254
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Electron device/Electronic equipment
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: Noda Minoru 京都工芸繊維大学, 電気電子工学系, 教授 (20294168)
• Project Period (FY): 2016-04-01 – 2017-03-31
• Keywords: バイオセンサー / Ａβ / オリゴマー / 動態

Outline of Final Research Achievements

Liposome biosensors are developed to detect dynamics of aggregation and fibrillization of amyloid-beta oligomer. Those sensors are expected to realize early and simple diagnosis and evaluation of progress in pathological change of Alzheimer Disease that expresses aggregation and accumulation of abnormal protein. In this research, both sensor device and biosensing phospholipid of liposome are investigated and developed as follows: I. Construction of the sensor device, II. Improvement in sensitivity of the sensor, and III. Comparison with ELISA result. A micro-TAS chip comprising of liposome cantilever sensor and microfluidic channels was fabricated. Consequently, dynamic aggregation and fibrillization of amyloid-beta related to its oligomer was selectively detected in human serum. Also, a high sensitivity was estimated that can detect less than 1 nM by excellent selection of phospholipid species, techniques of low-noise electronic circuit and precise temperature control of substrate.

Free Research Field

集積デバイス工学