A new method for immobilizing proteins on synthetic resins using supercriticalcarbon dioxide

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K14472
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Reaction engineering/Process system
• Research Institution: Osaka Prefecture University
• Principal Investigator: OGINO Hiroyasu 大阪府立大学, 工学(系)研究科(研究院), 教授 (80233443)
• Co-Investigator(Kenkyū-buntansha): 安田 昌弘 大阪府立大学, 工学(系)研究科(研究院), 教授 (40264808) ; 山田 亮祐 大阪府立大学, 工学(系)研究科(研究院), 准教授 (40608626)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 超臨界 / 二酸化炭素 / タンパク質 / プラスチック / 固定化

Outline of Final Research Achievements

Synthetic resins produced from various polymer compounds are used as materials for various products because they are inexpensive, highly durable, and easy to mold. On the other hand, it is difficult to firmly immobilize low-durability proteins on the surface of synthetic resins, because synthetic resins are highly durable. In this research, a new method for immobilizing proteins on the plastic surface was investigated using supercritical carbon dioxide which expands synthetic resins and improves the solubility of proteins and enzymes. In addition, the immobilization of directionally controlled proteins using peptide tags was also investigated.

Free Research Field

生物反応工学

Academic Significance and Societal Importance of the Research Achievements

本手法は、粒子、フィルム、あるいは微細管の内表面などあらゆる形状のプラスチック表面にタンパク質や酵素を固定化でき、プラスチックの表面改質や触媒機能付与が可能であるとともに、安価なバイオセンサーの作製も可能である。