Multi-layered interface of genes enables regulation of gene transfection timing

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14498
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Fujita Satoshi 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 研究グループ長 (00392655)
• Co-Investigator(Renkei-kenkyūsha): Sugiura Shinji 国立研究開発法人 産業技術総合研究所, 生命工学領域 創薬基盤研究部門 医薬品アッセイデバイス研究グループ, 上級主任研究員 (00392655)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 遺伝子導入 / 表面化学

Outline of Final Research Achievements

We attempted to make the interface of multi-layered genes where plasmids encoding different genes are transfected into cells by controlling timing. Plasmid expressing a fluorescent protein was encapsulated into cationic lipid to make cationic lipoplex. And multi-layered interface on glass slide was made by the cationic lipoplex, PLL and anionic hyaluronic acid. After seeding cells on the interface, we checked transfection timing of genes. As a result, we found transfection timing between the gene in upper layer and in lower layer is different for more than 20 hours.

Free Research Field

マイクロバイオプロセス工学