2017 Fiscal Year Final Research Report
Development of novel synaptic red chloride probe
| Project/Area Number |
16K14552
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Saitama University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 神経科学 / 神経活動 / 抑制性シナプス / クロライドプローブ / 蛍光イメージング |
|---|
| Outline of Final Research Achievements |
Increases in intracellular chloride ion concentrations of neurons following inhibitory postsynaptic potentials (IPSPs) have not been visualized in vivo to date. In this study, aiming at detecting IPSPs in vivo, we developed a red fluorescent genetically encoded chloride probe with high responsivity. Then we expressed the probe in neuronal cells and assessed its functionalities, focusing on responses to inhibitory inputs.
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| Free Research Field |
神経科学
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