2017 Fiscal Year Final Research Report
Development of induction method of insulin-producing cells in vivo
| Project/Area Number |
16K14588
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | インシュリン / β細胞 / 再生 / 転写因子 / 肝細胞 |
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| Outline of Final Research Achievements |
To search for new factors that promote conversion to insulin-producing cells from liver cells, Mouse insulin promoter 1-GFP transgenic mouse (MIP-GFP Tg), which has GFP reporter regulated by mouse insulin promoter was used. After isolating insulin producing cells, we did microarray analysis for identifing genes that are expressed in normal β cells, but not in induced insulin producing cells. As a result, new candidate genes Isl1β and Efl3 were identified. Each candidate gene was examined to confirm cooperative action in insulin production with Pdx1, NeuroD, MafA in vivo. Coexpression of Isl1β enhanced insulin production from liver cells by about 10 times. On the other hand, coexpression of Efl3 revealed that insulin transcription was suppressed contrary to expectation.
From the above results, it has been confirmed that Isl1β, together with Pdx1, NeuroD, MafA, has an effect of enhancing the production of insulin from liver cells. These results were published in Endocrinology.
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| Free Research Field |
分子生物学
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