Development of a mice having simian immune systems-Establishment of a new animal model for analysis of pathogenesis in viral infection

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14593
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Kyoto University
• Principal Investigator: Ibuki Kentaro 京都大学, 医学研究科, 准教授 (00273524)
• Co-Investigator(Kenkyū-buntansha): 三浦 智行 京都大学, ウイルス・再生医科学研究所, 准教授 (40202337)
• Research Collaborator: Sekine Sho ; Jinno Moe ; Yokoyama Haruka
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 小動物モデル / ウイルス感染症 / 免疫不全ウイルス / サル造血幹細胞 / 免疫不全マウス

Outline of Final Research Achievements

This study is aimed to establish a new animal model for AIDS. To attempt the development of mice having monkey immune cells (simianized mice), rhesus macaque hematopoietic stem cells (rHSCs) were transplanted into immunocompromised mice (NOG mice). After rHSCs transplantation, monkey CD4 positive T cells were persistently detected in mice peripheral blood. When these mice were infected with SIV (SIVmac239 or SIV PBj), SIVDNA / RNA was detected in the peripheral blood of mice, and the presence of the virus was also confirmed in these mice tissues. There were significant differences in pathogenicity between SIVmac239 or SIV PBj inoculated mice similar to those observed in monkeys in each SIV infection. From the above, it was suggested that the simian mouse may be used for the virus research including AIDS pathogenicity.

Free Research Field

ウイルス学