Experimental induction of structural variations in the mouse genome by breakage-fusion bridge cycle

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14609
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Osaka University
• Principal Investigator: KOKUBU CHIKARA 大阪大学, 医学系研究科, 准教授 (70379238)
• Co-Investigator(Renkei-kenkyūsha): SESE Jun 国立研究開発法人産業技術総合研究所, 創薬基盤研究部門, 主任研究員 (40361539)
• Research Collaborator: TANAKA Sachiyo 大阪大学, 医学系研究科, 特任研究員
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: がんゲノム / ゲノム工学 / マウスES細胞

Outline of Final Research Achievements

Recent advances in genome sequencing technologies revealed that complicated structural variations, which are assumed to be driven by chromosomal breakage-fusion bridge (BFB) cycle, occur in a variety of cancer genomes, although their carcinogenic implication has not been fully elucidated. In this study, we developed an experimental vector system to introduce BFB mutations in cultured mouse embryonic stem cells. By using this vector system, we also performed a genome-wide screening for regions that can undergo BFB mutations without losing their host cell viability. Interestingly, such regions showed characteristic features of genomic organization.

Free Research Field

遺伝学