Screening of no-coding RNA that can control transcription and chromatin

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14638
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Hokkaido University
• Principal Investigator: Murakami Yota 北海道大学, 理学研究院, 教授 (20260622)
• Co-Investigator(Renkei-kenkyūsha): TAKAHATA Shinya 北海道大学, 理学研究院, 助教 (50381588)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: non-coding RNA / 転写制御 / 分裂酵母

Outline of Final Research Achievements

To screen non-coding RNAs that have an ability to control transcription in fission yeast, we tried to construct a screening system using structure-specific RNA binding protein MS12 that enable tether non-coding RNAs to the promoter of reporter genes. However, we gave up to use MS12, because MS2 itself shows transcription stimulation activity. We next try to utilize CRISPR-dCAS9 to tether RNAs onto the promoter. We succeeded to recruit CRISPR-dCAS9 to the target region of fission yeast genome, but its efficiency is still low. We are trying to improve the efficiency.

Free Research Field

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