Development of new genome binning techniques using fusion PCR for genome-centric metagenomics.

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14646
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Sekiguchi Uiji 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ長 (20313570)
• Co-Investigator(Kenkyū-buntansha): ディーター トゥールース 国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (00598485)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: メタゲノム / 難培養微生物 / 未培養微生物 / 微生物ゲノム

Outline of Final Research Achievements

Genome-centric metagenomics is a key technique in state-of-the-art microbiome research. This method involves reconstruction of genome sequences from metagenomic reads using advanced bioinformatics algorithms and has been successfully applied for recovering tens to hundreds of population genome (genome bins, GBs). However, methodological advances remain needed to improve our ability to specifically recover GBs associated with populations of interest, identified using e.g., high-throughput 16S rRNA gene amplicon sequencing (16S-seq). Current techniques for genome binning are based mainly on genome signatures such as tetranucleotide frequencies and abundance, without taken into account information of the origin of the reads from specific phylotypes. We developed a new workflow that combines phylotype signatures with traditional metagenome binning strategies, which can significantly improve performance of genome-centric metagenomics.

Free Research Field

環境メタゲノミクス