Identification of microRNA editing related to the brains with Alzheimer's disease

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14649
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Medical genome science
• Research Institution: Niigata University
• Principal Investigator: Kuwano Ryozo 新潟大学, 脳研究所, フェロー (20111734)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: マイクロRNA / アルツハイマー病 / ヒト脳組織 / 神経系培養細胞 / 遺伝子発現

Outline of Final Research Achievements

To determine the epigenetic effects on the progression on Alzheimer’s disease (AD), the gene expression through microRNA in the brain was analyzed. All autopsied brains are systematically diagnosed with Braak staging based on the density of amyloid deposition and neurofibrillary tangles in the brain. The temporal cortex tissues were carefully dissected and stored at －80 °C until use. The expression of miR-501_3P was remarkably upregulated in the AD brains related to the pathological progression.
The overexpression of miR-501-3p in the neuroblastoma derived cultured cells (SH-SY5Y), which mimicked the miR-501-3p upregulation in the AD brains, resulted in the significant changes in the expression levels of 208 genes in which 128 genes were downregulated. Mutation in the seed sequence at the position 2 to 8 nucleotide of the miR-501_3P induced the significant alternative expression of the target mRNA in the SH-SY5Y cells.

Free Research Field

ゲノム医科学