Creation of self-evolving enzymatic system that enables semi rational design of genome

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14654
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: System genome science
• Research Institution: Kobe University
• Principal Investigator: NISHIDA KEIJI 神戸大学, 科学技術イノベーション研究科, 教授 (10620338)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: ゲノム編集 / 進化 / 人工進化

Outline of Final Research Achievements

The aim of this study is to develop self-evolving genomic system by using a modified DNA glycosylase which induce targeted random mutations at specific genomic locus. To characterize each DNA glycosylases from different sources, orthologs from yeast, bacteria, virus and plants were isolated, fused to CRISPR-dCas9 system and targeted. These were then expressed in yeast cells to assess specific mutagenesis at a genomic locus, showing distinct mutational spectra. By choosing one from these characterized, now region and speed of evolution can be controlled at more ordered manner.

Free Research Field

合成生物学