Development of 3D-gradient cultivation method for as-yet-uncultivated microorganisms growing in a narrow range of environmental conditions

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14663
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Conservation of biological resources
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: KATO Shingo 国立研究開発法人海洋研究開発機構, 次世代海洋資源調査技術研究開発プロジェクトチーム, 特任研究員 (40554548)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 分離培養 / 難培養微生物

Outline of Final Research Achievements

Isolation and cultivation of as-yet-uncultivated microorganisms are essential for basic and applied studies of microorganisms. However, most of microbial species is still as-yet-uncultivated. In the present study, I developed new cultivation methods to efficiently isolate not-yet-cultivated microorganisms. Using the methods, some novel species of iron oxidizers and ammonia oxidizers were successfully isolated or enriched in culture from natural environments. The new cultivation methods will contribute to discover and exploit novel useful microorganisms.

Free Research Field

微生物学