Proteomic mapping of Yb body in live OSCs via APEX tagging

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14666
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: The University of Tokyo
• Principal Investigator: Ishizu Hirotsugu 東京大学, 大学院理学系研究科(理学部), 助教 (40574588)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: piRNA / Yb body / RNA granule / APEX

Outline of Final Research Achievements

Eukaryotic cells contain many kinds of RNP granules; P bodies, stress granules, etc. Several observations suggest that liquid-liquid phase separation is the molecular basis for RNP granule assembly. Weak multivalent interactions between RNA-binding proteins and ssRNAs play a role in RNP granule assembly. Yb bodies are non-membrane-bound organelles consisting of precursor piRNA transcripts and piRNA factor proteins in Drosophila ovarian somatic cells, and are involved in piRNA biogenesis. However, the composition and function of Yb bodies are largely unknown.
Ascorbic acid peroxidese (APEX) has been used to interrogate specific protein networks. In this study, APEX was fused to Yb to capture components of Yb bodies in OSCs. We found that APEX proximity labeling was able to enrich protein components of Yb bodies.

Free Research Field

分子生物学