2017 Fiscal Year Final Research Report
Regulatory mechanism of homologous recombination in a Sex-chromosome-dependent manner
| Project/Area Number |
16K14668
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OHHATA Tatsuya 浜松医科大学, 医学部, 助教 (80616459)
NIIDA Hiroyuki 浜松医科大学, 医学部, 准教授 (20336671)
KITAGAWA Kyoko 浜松医科大学, 医学部, 助教 (20299605)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 相同組換え / X染色体不活性化 |
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| Outline of Final Research Achievements |
We noticed the possibility of "the efficiency of HR is lower in female ES cells compared to male". In this study, we tried to confirm it and further aimed to clarify the mechanism. First, to establish the same background male and female ES cells, we crossed JF1 mouse females with 129bc3 mouse male and successfully established 2 clones of XX (JF 1/129) ES, XY (JF 1/129) ES. The homologous recombination efficiency was measured using these clones. The male showed a tendency higher than females but it was judged that the colonies themselves with homologous recombination themselves were extremely small and it was not possible to accurately evaluate at the present time.
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| Free Research Field |
分子生物学
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