Identification of centrosomal long-noncoding RNA in human cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14673
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: National Institute of Genetics
• Principal Investigator: KITAGAWA DAIJU 国立遺伝学研究所, 分子遺伝研究系, 教授 (80605725)
• Co-Investigator(Renkei-kenkyūsha): Shiratsuchi Gen 国立遺伝学研究所, 分子遺伝研究系, 博士研究員 (80625533)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: lncRNA / 細胞分裂 / 次世代シークエンス / 紡錘体 / 中心体

Outline of Final Research Achievements

In eukaryotic cells, the formation of bipolar spindles is crucial for accurate segregation of chromosomes in mitosis, and therefore it must be strictly regulated for the maintenance of genome integrity. Long non-coding RNAs (lncRNAs) have been recently revealed to function in a wide range of biological phenomena including cell proliferation and tumorigenesis. Interestingly, the existence of spindle-bound RNAs and their importance in mitosis have been previously predicted in human. However, the lncRNAs that function in specific mitotic processes remain to be identified to date. In this study, we identified two uncharacterized human lncRNAs as novel regulators of mitotic spindle formation and centriole formation, using a combination of next-generation sequencing, bioinformatics and biochemistry.

Free Research Field

細胞生物学