2017 Fiscal Year Final Research Report
Development and demonstration of novel purification techniques for disease-specific superficial proteins
| Project/Area Number |
16K14680
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | プロテオミクス解析 / 質量分析 / 表在性蛋白質 / 抗原探索 / 非天然ビオチン化ラベル / 改変型ストレプトアビジン / CTC / 血管内皮 |
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| Outline of Final Research Achievements |
Regarding the labeling reagent, succeeded in synthesizing a derivative in which a succinimide group (NHS group) was added to the non-natural biotin derivatives.
As a result of experiments and proteomics analyses of vascular endothelium using mouse, it was found that the surface protein were only purified and the endogenous biotinylated proteins could be excluded by using the new derivatives. Proteomic analysis was also performed on diseased mice seeded with A20 lymphoma. Analyses of the vascular endothelial proteins that increased by 1.5 times or more in tumor-bearing mice were performed. There are 147 kinds of proteins listed only by the conventional method, and 28 kinds of proteins commonly listed in the conventional method and the novel method. However, it turned out that there were 53 kinds of proteins that were only found to be enhanced by the novel method. Validation is now under study.
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| Free Research Field |
構造生物化学
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