Development of a high resolution three-dimensional structure analysis method of minimal molecules by KAGOME DNA origami

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14682
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Osaka University
• Principal Investigator: KATO Takayuki 大阪大学, 生命機能研究科, 特任准教授(常勤) (20423155)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: クライオ電子顕微鏡 / 構造解析 / DNA origami

Outline of Final Research Achievements

In this stady, we will develop a technique for fixied a small molecule in the center of KAGOME pattern DNA origami.It can be used as nano chamber for structural analysis of a small molecule, less than 100 kDa, by electron cryomicroscopie. The stator protein MotA in flagella motor was selected as target protein for evaluation for this techinique. In beginning, The MotA structure was solved by electron microscopie, and shape and size information was obtained. Then KAGOME pattern DNA origami was designed to fit it, which will formed by four single stranded DNA. The one DNA has biotin labeled for fixed the target protein into center of KAGOME DNA origami. The formation of KAGOME pattern was evaluated by electrophoresis and negative stained electron microscope.

Free Research Field

構造生物学