The stringent response in animals

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14694
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Masuda Shinji 東京工業大学, バイオ研究基盤支援総合センター, 准教授 (30373369)
• Co-Investigator(Kenkyū-buntansha): 鈴木 崇之 東京工業大学, 生命理工学院, 准教授 (60612760)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 緊縮応答 / 栄養応答 / ppGpp / ショウジョウバエ

Outline of Final Research Achievements

We previously established the quantification method for detecting ppGpp in plant tissues by use of LC-MS/MS technology. Here, we re-established the method to detect ppGpp in other organisms. As a results, we could detect some ppGpp not in plant tissues, but also algae and other organisms. We also try to express a bacterial ppGpp synthase in animal cells. We succeeded to obtain recombinant organisms that express the ppGpp synthase in different tissues. We found that over-production of ppGpp in the organisms results in significant behavioral responses in the organisms. This suggest that ppGpp-dependent stringent response could, at least, be operated in animals.

Free Research Field

分子生物学