2017 Fiscal Year Final Research Report
Quality control system for the newly synthesized tail-anchored proteins.
| Project/Area Number |
16K14699
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | BAG6 / ユビキチン / プロテアソーム / テイルアンカー蛋白質 / タンパク質品質管理 / タンパク質分解 / タンパク質凝集 / シグナル配列 |
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| Outline of Final Research Achievements |
A portion of newly synthesized tail-anchored proteins tend to fail their correct assembly into the lumen of endoplasmic reticulum, thus resulting in the production of the defective species. Although the efficient degradation of these aggregation-prone polypeptides is crucial, the molecular mechanism of their elimination pathway has not been adequately characterized. In this study, we focused on one such cryptic portion of the defective transmembrane domain protein, and show that a part of these is produced as a labile species that is immediately targeted to the degradation pathway. We found that proteasomes are indispensable for elimination of mislocalized tail-anchored species. These observations suggest that protein degradation machinery acts as a critical factor for degradation of mislocalized tail-anchored proteins.
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| Free Research Field |
細胞生物学、生化学
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