Measure the temperature of specific biomolecules in cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14705
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: Funatsu Takashi 東京大学, 大学院薬学系研究科(薬学部), 教授 (00190124)
• Co-Investigator(Renkei-kenkyūsha): OKABE Kohki 東京大学, 大学院薬学系研究科, 助教 (20455398)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: ナノバイオ / 細胞・組織 / 生体分子 / 分析科学

Outline of Final Research Achievements

The Raman spectrum of O-H stretching vibration of water molecule is broad, and it is well known that two peaks are observed and that the isosbestic point is 3419 cm-1. As the temperature gets higher, the peak on the low Raman shift side becomes smaller and the peak on the high Raman shift side becomes larger, so we could measure the temperature using this property. We also measured Raman spectra of low frequency oscillations such as C-C-N bending vibration (458 cm-1) and Te-Te stretching vibration (150 cm-1) of diphenyl ditelluride. We could measure the temperature in the microscopic region (in the order of micrometers) from the ratio of Stokes and anti-Stokes light intensities.　It is expected that Raman temperature measurement within a cell will be possible by synthesizing a probe combining a compound exhibiting low frequency Raman oscillation and a compound accumulating in specific organelle.

Free Research Field

生物物理学