Quantitative analysis of ATP dynamics in photosynthetic organisms by a novel bioluminescence ATP biosensor

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14709
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Kyoto University
• Principal Investigator: Imamura Hiromi 京都大学, 生命科学研究科, 准教授 (20422545)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 生物発光 / BRET / イメージング / 光合成 / ATP

Outline of Final Research Achievements

We developed a novel genetically encoded BRET-based ATP biosensor, BTeam. It reacts specifically to ATP, and reports ATP concentrations in a range of 0.5 - 6.0 mM as BRET efficiencies. By measuring BRET efficiencies of BTeam-expressing cultured cells, we were able to quantitatively estimate ATP concentrations inside living cells. We also demonstrated that BTeam can report ATP concentrations inside a living organism using a zebrafish expressing BTeam. Currently, we are constructing a plant expressing BTeam in order to monitor ATP dynamics in plants during photosynthesis.

Free Research Field

バイオイメージング、生物物理学、生化学