Dynamism of a proton-driven protein translocation motor.

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K14713
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Tsukazaki Tomoya 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (80436716)
• Co-Investigator(Renkei-kenkyūsha): TANAKA Yoshiki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (10632333)
• Research Collaborator: FURUKAWA Arata ; SUGANO Yasunori
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: エックス線結晶構造解析

Outline of Final Research Achievements

The protein translocation across the membrane is one of the fundamental, conserved reactions in all cells. SecDF, a proton driven motor, drives the protein translocation. SecDF has transmembrane helixes and soluble domains. Although the soluble region was shown to be highly mobile, the details were not clear. In this study, we have determined the crystal structures of SecDF that are different from the previous report. the data and the subsequent functional analyses, we propose a functional model of SecDF.

Free Research Field

構造生物学