The molecular mechanism of synchronization of oscillatory gene expression between cells in the segmentation clock

2016 Fiscal Year Final Research Report

• Project/Area Number: 16K14734
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: Kageyama Ryoichiro 京都大学, ウイルス・再生医科学研究所, 教授 (80224369)
• Research Collaborator: KOBAYASHI Kumiko ; ISOMURA Akihiro , さきがけ研究員
• Project Period (FY): 2016-04-01 – 2017-03-31
• Keywords: 分節時計 / 遺伝子発現振動 / 同期化 / ライブイメージング / 光遺伝学 / Notchシグナル / Hes7 / Delta-like1

Outline of Final Research Achievements

We generated transgenic mice carrying a new Hes7 reporter, and succeeded in live imaging of oscillatory expression at the single-cell resolution in the segmentation clock. We also generated cells in which the expression of the Notch ligand Delta-like1 (Dll1) is induced by an optogenetic method. By using these tools, it is now possible to perform live-imaging analysis of the effect on cells by optogenetic perturbation of oscillation to analyze the mechanism of synchronization of oscillatory gene expression between cells in the segmentation clock.

Free Research Field

分子生物学