Water volume regulation involved in primaly blood production

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K14739
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Kyushu University
• Principal Investigator: Sato Yuki 九州大学, 医学研究院, 准教授 (90508186)
• Research Collaborator: SHIGEMATSU mugiho
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: アクアポリン / 血管内皮細胞 / 内皮ー造血転換 / 二次造血

Outline of Final Research Achievements

Vascular endothelial cells and blood cells differentiate from common progenitor cells. Both are spatially separated, and blood cells circulate in the blood vessels as the heart starts to beat. Although the molecules involved in the blood and blood vessel differentiation have been detailed, how and when the water of the first appearing blood is taken into the vasculature have not been addressed. In this study, we focused on the water channel molecule Aquaporin1 (AQP1) and analyzed it in order to elucidate the inflow mechanism of water into the blood vessels at the early embryonic development. We found a new possible mechanism by which AQP1-mdeiated selective water influx promotes morphological transition from flat endothelial cells to spherical blood cells.

Free Research Field

発生生物学

Academic Significance and Societal Importance of the Research Achievements

内皮ー造血転換の際に観察される扁平（内皮）から球形（血球）の細胞形態変化が一体どのようなしくみによって引き起こされるのかは、これまで未解明であった。本研究から、水チャネルAQPを介しておこる細胞内水流入が細胞の球状化に関わることが明らかになった。水分子の流入制御の観点から血液産生メカニズムを解明しようとした研究はこれまでになく、内皮ー造血転換の分子機構解明に向けて新たな突破口となることが期待される。